fopflash construct Search Results


topflash/fopflash reporter constructs for measuring tcf/lef activity in the beta-catenin signaling pathway  (Merck KGaA)
90
Merck KGaA topflash/fopflash reporter constructs for measuring tcf/lef activity in the beta-catenin signaling pathway
Topflash/Fopflash Reporter Constructs For Measuring Tcf/Lef Activity In The Beta Catenin Signaling Pathway, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fopflash+construct/pmc06684922-58-9-15?v=Merck+KGaA
Average 90 stars, based on 1 article reviews
topflash/fopflash reporter constructs for measuring tcf/lef activity in the beta-catenin signaling pathway - by Bioz Stars, 2026-06
90/100 stars
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topflash/fopflash firefly and sv-40 renilla luciferase constructs  (BioVector Inc)
90
BioVector Inc topflash/fopflash firefly and sv-40 renilla luciferase constructs
Topflash/Fopflash Firefly And Sv 40 Renilla Luciferase Constructs, supplied by BioVector Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fopflash+construct/pm28272338-264-11-21?v=BioVector+Inc
Average 90 stars, based on 1 article reviews
topflash/fopflash firefly and sv-40 renilla luciferase constructs - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
fopflash construct  (Merck & Co)
86
Merck & Co fopflash construct
Smurf1 promoted Wnt/β-catenin signaling activation. ( A ) Parental and Smurf1-overexpressing AGS cells were transfected with TOPflash and <t>FOPflash</t> reporters and treated with Wnt3a, respectively. Wnt/β-catenin signaling activation was assessed using the TOPflash/FOPflash dual luciferase reporter system. Relative luciferase units (RLU) were calculated to assess β-catenin-triggered transcription. After <t>Smurf1</t> <t>overexpression</t> in AGS cells, cytoplasmic ( B and D ) and nuclear ( C and D ) β-catenin protein levels were measured using the western blot assay. β-actin and Lamin B1 served as cytoplasmic and nuclear markers, respectively. ( E ) After Smurf1 overexpression in AGS cells, β-catenin nuclear translocation was assessed using IF analysis. Red fluorescence indicated β-catenin, and blue fluorescence indicated the nucleus. The results were shown as the median (1st quartile and 3rd quartile). * p < 0.05, ** p < 0.01.
Fopflash Construct, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fopflash+construct/pmc12630608-57-14-16?v=Merck+%26+Co
Average 86 stars, based on 1 article reviews
fopflash construct - by Bioz Stars, 2026-06
86/100 stars
  Buy from Supplier

Image Search Results


Smurf1 promoted Wnt/β-catenin signaling activation. ( A ) Parental and Smurf1-overexpressing AGS cells were transfected with TOPflash and FOPflash reporters and treated with Wnt3a, respectively. Wnt/β-catenin signaling activation was assessed using the TOPflash/FOPflash dual luciferase reporter system. Relative luciferase units (RLU) were calculated to assess β-catenin-triggered transcription. After Smurf1 overexpression in AGS cells, cytoplasmic ( B and D ) and nuclear ( C and D ) β-catenin protein levels were measured using the western blot assay. β-actin and Lamin B1 served as cytoplasmic and nuclear markers, respectively. ( E ) After Smurf1 overexpression in AGS cells, β-catenin nuclear translocation was assessed using IF analysis. Red fluorescence indicated β-catenin, and blue fluorescence indicated the nucleus. The results were shown as the median (1st quartile and 3rd quartile). * p < 0.05, ** p < 0.01.

Journal: Scientific Reports

Article Title: Smurf1 promotes gastric cancer progression by regulating Axin2-dependent Wnt signaling pathway

doi: 10.1038/s41598-025-23707-3

Figure Lengend Snippet: Smurf1 promoted Wnt/β-catenin signaling activation. ( A ) Parental and Smurf1-overexpressing AGS cells were transfected with TOPflash and FOPflash reporters and treated with Wnt3a, respectively. Wnt/β-catenin signaling activation was assessed using the TOPflash/FOPflash dual luciferase reporter system. Relative luciferase units (RLU) were calculated to assess β-catenin-triggered transcription. After Smurf1 overexpression in AGS cells, cytoplasmic ( B and D ) and nuclear ( C and D ) β-catenin protein levels were measured using the western blot assay. β-actin and Lamin B1 served as cytoplasmic and nuclear markers, respectively. ( E ) After Smurf1 overexpression in AGS cells, β-catenin nuclear translocation was assessed using IF analysis. Red fluorescence indicated β-catenin, and blue fluorescence indicated the nucleus. The results were shown as the median (1st quartile and 3rd quartile). * p < 0.05, ** p < 0.01.

Article Snippet: After Smurf1 overexpression, AGS cells were co-transfected with TOPflash construct (80 ng, Merck) or FOPflash construct (Merck) and pRL-TK plasmid (8 ng, Promega, WI, USA) using PEI max transfection reagent.

Techniques: Activation Assay, Transfection, Luciferase, Over Expression, Western Blot, Translocation Assay, Fluorescence